Serial dilution of TTX (tetrodotoxin)?

It depends on what degree of dilution you need and how much. If you need 1ml of each dilution then you need to work out whether to go to 0.01, 0.001 ect or perhaps 0.1, 0.05, 0.001 etc As soon as you know your dilutions and you know how much you need of each then say it is the first example I have mentioned then you use the equation c1V1=c2v2 where 0.01* 1ml= 0.1*= ? and then make this your next standard and dilute this one rather than always from your original stock, Its better to do it in implemants. Perhaps get a chem book of solutions and dilutions to go over this as it can feel confusing but it is basically simple math and practise.